The nucleotide sequence of the glucoamylase gene GLA1 from Saccharomycopsis fibuligera KZ
نویسندگان
چکیده
منابع مشابه
Structure of glucoamylase from Saccharomycopsis fibuligera at 1.7 A resolution.
The yeast Saccharomycopsis fibuligera produces a glucoamylase which belongs to sequence family 15 of glycosyl hydrolases. The structure of the non-glycosyl-ated recombinant enzyme has been determined by molecular replacement and refined against 1.7 A resolution synchrotron data to an R factor of 14.6%. This is the first report of the three-dimensional structure of a yeast family 15 glucoamylase...
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We isolated two genes for extracellular beta-glucosidase, BGL1 and BGL2, from the genomic library of the yeast Saccharomycopsis fibuligera. Gene products (BGLI and BGLII) were purified from the culture fluids of Saccharomyces cerevisiae transformed with BGL1 and BGL2, respectively. Molecular weights of BGLI and BGLII were estimated to be 220,000 and 200,000 by polyacrylamide gel electrophoresis...
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Cell-wall-associated and extracellular alpha-glucosidases were purified to homogeneity from Saccharomycopsis fibuligera KZ growing on a medium containing cellobiose as the sole source of carbon; this substrate has the greatest inducing effect on the production of both forms of the enzyme. Depending on the source of carbon, 75-90% of the enzyme is associated with cell wall, from which it can be ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1991
ISSN: 0378-1097
DOI: 10.1016/0378-1097(91)90452-g